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Implications of AMPK in the formation of epithelial tight junctions

Quantitative polymerase chain reaction (qPCR) was performed using SsoAdvanced universal SYBR green supermix (Bio Rad), 5 ng genomic DNA, 10 M primers (mMitoF1: 5 CTA GAA ACC CCG AAA CCA AA 3, mMitoR1: 5 CCA GCT ATC ACC AAG CTC GT 3, mB2MF1: 5ATG GGA AGC CGA ACA TAC TG 3 and mB2MR1: 5 CAG TCT CAG TGG GGG TGA AT 3 3,93 and RNAse-free water in a 25 L reaction volume on a CFX96 opus 96 Real-Time PCR system (Bio-Rad)

PMID 34463268

Some studies suggest ubiquinol may have better absorption in certain populations, particularly older adults, though individual responses vary