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Long-term and therapeutic-level hepatic gene expression of human factor IX after naked plasmid transfer in vivo

Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later

The levels of the substance decline with age, and that is why people turn to supplements to improve their health
