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Once reaching near-confluence, culture medium was aspirated and cells were washed once with PBS, followed by a 20 min incubation with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei
Higher serum trimethylamine-N-oxide levels are associated with increased abdominal aortic calcification in hemodialysis patients
