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N.IversonT
Partitioning of -SNAP in Triton X-114 To assess the amphipathic nature of -SNAP, Triton X-114 phase partitioning was performed following a protocol described previously 63
Equal amounts of protein were loaded on SDSpolyacrylamide gel electrophoresis (SDSPAGE), using Mini-PROTEAN TGX gels (Bio-Rad, 4561096) and transferred to a 0.2-mm nitrocellulose membrane (Bio-Rad, 1620112)
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