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For quantitative PCR (qPCR), total RNA was reverse transcribed into complementary DNA using a Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1682) following the manufacturers instruction

Katie is the 14 year old youngster whose situation was outlined earlier in this article

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doi: 10.1523/JNEUROSCI.0392-12.2012 334 TsaiH.-H.FrostE.ToV.RobinsonS.ffrench-ConstantC.GeertmanR.et al
