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Off-target toxicity also poses a significant hurdle

Kumnovics A, Chen OS, Li L et al (2008) Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial ironsulfur cluster synthesis

The way to avoid this pitfall is by identifying possible obstacles to achieving your goal, and thinking two or three steps ahead

doi: 10.1016/j.dld.2011.01.018
