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On the other hand, diosgenin and charantin are the steroidal sapogenins having higher lipid solubility, hence have least volume of distribution, which affects their bioavailability

Moreover, the study shows that in vitro over-expressing of -klotho in human proximal tubular cells decreases IGF-1-induced AKT phosphorylation, indicating that the inhibitory effects of -klotho on compensatory renal hypertrophy may be mediated, at least in part, through reduced IGF-1 signaling.28 Notably, inhibition of the GH receptor by the specific antagonism G120K-PEG blocks renal IGF-1 upregulation and subsequent kidney hypertrophy in Uni-Nx animals

However, injection carries its own risks (infection, sterility, injection site reactions)

Vitamin C Vitamin C is the antioxidant and brightener