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Then, membranes were incubated with primary antibodies against NOS2 (1:1000, ABclonal, Wuhan, China), NOX1 (1:1000, ABclonal, Wuhan, China), HO-1 (1:1000, ABclonal, Wuhan, China), SOD2 (1:1000, ABclonal, Wuhan, China), Kelch-like ECH-associated protein 1 (KEAP1) (1:1000, Proteintech, Wuhan, China), nuclear factor B (NF-B) P65 (1:1000, Cell Signaling Technology, Danvers, USA), GAPDH (1:10000, Proteintech, Wuhan, China), Histone H3 (1:4000, HUABIO, Hangzhou, China) and Tublin- (1:10000, abcam, USA) at 4 C overnight followed by incubation with secondary antibody (1:5000, Proteintech, Wuhan, China) for 1 h at RT

K., Anantharamaiah G

Agisha et al., Pseudomonas fluorescens to the phyllosphere of an apple scab-susceptible apple ( Malus domestica ) cultivar led to the up-regulation of genes encoding proteins participating in pathogen recognition, signaling and antimicrobial defense such as PR-proteins, thioredoxin-like proteins, heat shock proteins and a GST (Krkcoglu et al., 2007)

doi: 10.3390/nu9010029