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diluted 1:5000) for 1 h at room temperature in 5% milk in 1 TBS + 0.1% Tween 20, bands were visualized using ECL solution (GE Healthcare, RPN2106, Thermo Fisher Scientific, cat#80196) and calibrated using a ChemiDoc Imaging System (Bio-Rad, Hercules, CA, USA)
Emerging or marginal themesKeywords like CKD, renal function, and I/R injury show minimal connectivity, suggesting limited integration within the broader network
