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After 28 days, the solution should be discarded and a fresh vial reconstituted if ongoing research requires it
In the in vivo experiments, the proportion of M1 macrophages was reduced both two and four weeks after surgery at the tendon-bone interface
This dysfunction manifests as reduced vascular response to dilators (particularly to NO and prostacyclin), heightened pro-inflammatory responses leading to vascular wall inflammation, increased oxidative stress contributing to vascular damage, and pathological changes
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