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The incubation was carried out for 30 minutes with continuous shaking at 30C, and the flasks were continuously gassed with a mixture of 95% O 2 and 5% CO 2
[DOI] [PMC free article] [PubMed] [Google Scholar] 1070.Kappelmann N., Khandaker G.M., Dal H., Stochl J., Kosidou K., Jones P.B., Dalman C., Karlsson H
After mixing the two bacteria cultures, the mixture is concentrated before being seeded onto NGM agar plates
We consider that the expression of SIRT1 might be also regulated by microRNA (miRNA) in auditory cells, based on the results of previous studies 60,61,62,63
