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Other destructive approaches include focused ultrasound [65] , CO 2 laser, radiofrequency ablation [66] , photodynamic therapy [67,68] , Er:YAG or Nd:YAG laser [69] , and electrodessication [70]

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Varying amounts of monobromobimane stock solution (0-300 l) were added to respective cuvettes already containing 5 l of the enzyme solution (protein concentration of 810 g ml -1 ), glutathione (100 l of stock solution) and phosphate buffer (3.0 ml)