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Common symptoms include: Persistent fatigue Low energy levels Shortness of breath Dizziness or feeling faint Irregular heartbeats (palpitations) Pale or yellowish skin Reduced appetite or unintentional weight loss If left untreated, B12 deficiency can lead to serious complications, including nerve damage, cognitive issues, and changes in mood

The precursor provides raw material for NAD+ synthesis while 5-amino-1mq prevents NNMT from draining the nicotinamide pool

Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample