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Description
It consists of three amino acids: cysteine, glutamic acid, and glycine

ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer

RNA (300 ng) and reverse transcribed with SuperScript III (Invitrogen, Carlsbad, CA) using 150 ng of random hexamers, 10 mM deoxynucleoside triphosphates (dNTPs), and 40 U of RNaseOut (Invitrogen)

This article breaks down each method, compares their strengths and limitations, and outlines best practices for sample handling that prevent artifactual oxidation from compromising data integrity