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Under physiological steady-state conditions, the body meticulously regulates the equilibrium between copper intake and elimination to preserve optimal copper concentrations

Begin with alternate-day application and progress to daily use after two weeks without irritation

To Spraque-dawley mice short-term give big dose dextran iron (100 mg/kg of iron 7 days), liver of animals to dissect histologic study, found in the liver cells appear in the iron deposition, but did not see the damage to the liver cells

This was demonstrated using Nrf2/ mice and N-acetylcysteine administration prevented these changes (Thimmulappa et al., 2006)