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doi: 10.1046/j.1365-2133.146.s61.2.x
An interaction of behavioral and physiologic factors is thought to cause long-standing functional constipation with overflow incontinence
After cooling, acid methylation with 6 N HCl in 50% (v/v) methanol (2 ml) was performed for 10 min at 80C followed by immediate cooling on ice
