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Stockwell and co-workers found that depletion of GSH by erastin inactivated GPX enzymes and induced ferroptosis [21]

Rapid Oxidation: Free glutathione quickly oxidizes into glutathione disulfide (GSSG) upon exposure to air or light, losing its efficacy and causing discoloration (browning) of the product

M., Fischer, T

After electrophoresis, protein bands were visualized by Coomassie Brilliant Blue R-250 staining