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These in vitro studies in monolayers with cancer-derived cell lines differ radically from the situation in vivo in the normal intact intestinal epithelium with numerous specialized cell types, intact blood and lymphatic circulations, and other structures and factors such as the glycocalyx, secreted mucus, reticuloendothelial cells, other immune cells, and mesenteric nerves

Despite three decades of preclinical research, not a single human clinical trial exists for mental health applications

All other evidence is from rodent and in vitro models