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Western blot analysis using 3-nitrotyrosine specific antibodies to detect tyrosine nitration of peroxynitrite-treated GSNOR protein did not show any nitration (data not shown) indicates that the peroxynitrite-dependent loss of GSNOR activity is probably due to the oxidation of cysteine(s) (Zeida et al., 2013)

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Getting consistent, high-quality sleep allows your body to enter a state of repair where it can recycle oxidized glutathione back into its active, reduced state