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Description
Meighan, et al

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EHMES-1 cells were stimulated by the respective dimeric macrocycles and the phosphorylation level was quantified by enzyme-linked immunosorbent assay (ELISA) using a rabbit pY1234/1235-specific mAb

In brief, 20 l of Viability/Cytotoxicity Reagent containing both GF-AFC was added to each well, followed by 30 sec mixing at room temperature and incubation at 37C for 30 min
