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After 15 min agitation at 8 C followed by 15 min centrifugation at 20800 g and 4 C, the supernatant was collected in a 15 ml Falcon tube and protein content was measured using the DC TM Protein Assay (Biorad, Feldkirchen, Germany)

In addition, studies have shown that LNP formed by ethanol dilution/rapid mixing with aqueous media give rise to structures that can be modelled according to the lipid composition 18