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Description
The LIP is dynamically set by transferrin/TfR1 uptake (iron import), ferritin (safe storage via H-chain ferroxidase activity), NCOA4-mediated ferritinophagy (ferritin degradation releasing Fe), heme turnover by HO-1 (which can paradoxically raise LIP if not matched by storage/export) and mitochondriallysosomal exchange routes that shuttle iron for heme/FeS biogenesis
The beads were washed to get rid of free ligand with coupling buffer, and remaining active chemical groups blocked by 1 M ethanolamine at pH 8.0
Phenazines affect biofilm formation by Pseudomonas aeruginosa in similar ways at various scales
December 2012, 22(12): 1294-1295
