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Description
These enzymes insert Co 2+ into sirohydrochlorin to generate Co(II)-sirohydrochlorin but are structurally related to the iron-inserting enzyme SirB that converts sirohydrochlorin into siroheme [157,163,164], as well as to CbfA, which is the nickel chelatase that inserts Ni(II) into sirohydrochlorin for coenzyme F 430 biosynthesis [165,166]

And it makes it very hard to to apply a blanket rule - some people may manage well with alcohol and injections

Engineering toward a Bacterial Endoplasmic Reticulum for the Rapid Expression of Immunoglobulin Proteins

Recent evidence shows that disruption of the flux through the pathway has deleterious consequences
