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Expression of pluripotency markers Nanog homeobox ( NANOG ), octamer-binding transcription factor 4 ( OCT4 ), reduced expression 1 ( REX1 ), and SRY-box transcription factor 2 ( SOX2 ) significantly decreased in both iNs_D7 and iNs_D14 compared to iPSCs (Fig

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Additionally, the oxidative degradation of PUFAs generates two secondary lipid peroxides, 4-hydroxy-2-nonenal (4-HNE) and malondialdehyde (MDA), which are highly expressed during the onset of ferroptosis and serve as markers of this process