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Reagent 4

Setria - Glutathione high bioavailability

If none of your protein, or very little, is in the flow-through during your purification, then youve used sufficient glutathione agarose (Figure 5, left)

The tripeptide glutathione is found in all eukaryotic cells, and due to the compartmentalization of biochemical processes, its synthesis takes place exclusively in the cytosol