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Description
In situ hybridization In situ hybridization (ISH) was performed on fresh frozen coronal brain sections (25 m) according to protocols that are available at Briefly, digoxigenin (DIG)-labeled RNA probes were transcribed in vitro from cloned cDNAs for mouse Pdgfra, Enpp6, Plp, Mbp and Mog as previously described (Huang et al., 2018
[DOI] [PubMed] [Google Scholar] 38.Prutz W.A., Monig H., Butler J., Land E.J
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