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Protein isolation and MS spectral-data acquisition Mixed BAL cells: BAL cells were resuspended in hypotonic lysis buffer with HALT protease inhibitor cocktail (Thermo Fisher Scientific), and lysed using sequential cell disruption techniques including a freezethaw at 98 C with vortexing and sonication (Sonics) on ice before buffering with 1 M triethylammonium bicarbonate (Sigma)

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c , Western blot determination of the EGFR levels in SKOV3 and Hep3B cells treated with indicated concentration of FR-Ctx (FAR 15)

Drugs are incorporated during the liposome formation process through passive loading (direct incorporation) or active loading (using pH gradients or other methods)