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One ml aliquot of the supernatant was neutralized with 1.5 ml of 0.5 M phosphate buffers (pH 7.5), then 50 l H 2 O was added
I should be applied every other evening before going to bed for the first 2 to 3 weeks, slowly increasing to every evening
4.1 Potential targets for drug development The molecular systems architecture of asthma identifies candidate molecular targets that warrant further experimental validation for potential drug development