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Next, brain tissue was lysed in PBS containing 200 mM n -octyl-beta-d-glucopyranoside and protease inhibitor cocktail (Roche) as described previously (Teesalu et al, 2009) with slight modifications

The determination kits of hepatic triglyceride (TG) and malondialdehyde (MDA) were provided by Apply-gen Technologies Corporation Ltd

308 In vitro studies have shown that the addition of S1P to microglia subjected to oxygen-glucose deprivation/reperfusion exacerbates hypoxia-induced neuronal apoptosis

The screening and confirmatory assay cut points were calculated from 51 healthy donor serum samples in accordance with regulatory guidance