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Air bubbles reduce the actual volume of water you transfer, creating the same concentration error described above

Baseline B12 Status and Dosage Selection Serum B12 levels below 300 pg/mL typically warrant higher starting doses (1,0001,500 mcg weekly), while levels 300500 pg/mL may benefit from lower initiation (500800 mcg)

10.1021/acs.biochem.6b00733 148 Van HarenM
Cellular assays: Investigating effects on cellular metabolism, mitochondrial function, and experimental metabolic models
