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To explore the impact of ArfGAP1 phosphorylation on this Arf1-dependent activity, we evaluated Golgi morphology in human SH-SY5Y neural cells overexpressing ArfGAP1 phospho-site variants by substituting either uncharged hydrophobic phospho-null (Alanine: A) or negatively-charged phospho-mimicking (Aspartic Acid: D) residues at Ser284, T291 and T292

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