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Cells were harvested and lysed in a buffer containing 20 mM Tris pH 8, 150 mM NaCl, 1% glycerol, protease inhibitor complex (Sigma), and 30 mM DDM
Smoothened Agonist (SAG) can effectively activate the sonic hedgehog (Shh) signalling pathway to promote the differentiation of ADMSCs into NP cells

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ISSN:1473-0189
