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Furthermore, the inclusion of the Klotho protein can enhance the formation of the ULK1 complex transcription factor EB nuclear translocation and block the IGF1/PI3K/Akt/mTOR signaling pathway, all of which are essential for autophagy activation [150]
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Network analysis revealed several metabolites exhibiting high connectivity, including S-adenosylhomocysteine, 5-methylthioadenosine, and diaminopimelic acid, reflecting their central roles within metabolic pathways rather than differential abundance between groups (Fig
