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T-cell subsets were analyzed by staining with antibodies to CD4, CD8, and CD25

Cell death LEC were trypsinized 48 h post transfection and incubated with 2 g/mL propidium iodide (PI) (P4170, Sigma)

Additionally, the long-term fate and metabolic impact of trace elements such as Ga and Se warrant investigation

The role of probiotics and prebiotics in modulating of the gut-brain axis
