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Description
If you have not yet done this protocol, you will find this protocol is NOT EASY
showed that tryptophan residues of carbonic anhydrase, carboxypeptidase A, alpha-chymotrypsin, trypsin, pepsin, and bovine and human serum albumins are characterized by the remarkable freedom of motion within the protein matrix, which implies that these matrices are highly flexible on the nanosecond time scale (Lakowicz et al., 1980)
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Bacterial proteinase method for the determination of the ratio of denaturation of globular proteins
