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Similar content being viewed by others Introduction Liver fibrosis, characterized by the excessive extracellular matrix (ECM) accumulation and impaired liver function, represents a progressive pathological condition underlying various chronic liver diseases

Organoids were transferred to a 6 well-plate on a CO 2 resistant orbital shaker (Thermo Scientific) and grown in differentiation medium consisting of DMEM:F12 supplemented with 1X N2 supplement, 1X B27 supplement, 1X penicillin (100 units/mL)/streptomycin (100 g/mL), 0.1mM 2-mercaptoethanol, 1X NEAA, and 2.5 g/mL human insulin

Taken together, the activation of oxidative and inflammatory pathways in cerebral endothelial cells provides a crucial route for translating peripheral inflammatory and oxidative stress signals through the BBB into the brain parenchyma