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RNC-NAC-NMT1 in vitro reconstitution for cryo-EM 100 nM RNCs were incubated with 135 nM wildtype NAC and 135 nM GST-NMT1 in Reconstitution Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.05% octaethylene glycol monododecyl ether, 100 mM sucrose) for 1 h on ice before directly preparing cryo-EM grids

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In vitro models fail to fully recapitulate the complexity of human ocular diseases, leading to inconsistent translational outcomes

At week 12, women in the collagen group displayed significantly improved skin scores for wrinkle, elasticity, hydration, radiance, and firmness compared with placebo control values (via VISIA skin analysis system, Cutometer , and Skin Quality Visual Analogue Scale)