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Numerous reviews and research articles mention analytical details, protocol variations and innovations like changing the reaction monitoring from 515 to 580 nm in order to minimise interferences due to carotenoids [94], separation from radicals of the compounds tested in order to avoid spectral overlap by means of HPLC-PDA detectors [95] or HPLC postcolumn configuration that allows the estimation of the total antioxidant activity of the individual compounds in a mixture [96,97]

It happens across every biological domain simultaneously

Results Establishment of the two experimental progenies throughout F1, F2 and F3 generations After the transfer of 158 vitrified-thawed embryos of 13 donors into 13 foster mothers (100% pregnancy rate), 69 animals were generated (vitrified-transferred group