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This method involves four main steps: isolation by strong cation-exchange SPE, mild derivatization with pentafluorophenyl trifluoromethanesulfonate, chromatographic separation using sequential ion-exchange/reversed-phase (ultra) high-performance liquid chromatography [(U)HPLC], and detection by electrospray ionization MRM-MS

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An improved understanding of the factors predisposing to PD, including genetic factors, and the links between ED and other comorbid conditions in humans, including malignancy, is necessary to better inform patient risk stratification, as well as long- and short-term approaches to health maintenance