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Cell volume measurements were taken over 60 s, and distributed in 200 bins spanning a volume range of 5006000 fL

[] Although the clinical manifestations caused by the toxic effects of various heavy metals are fairly diverse and commonly non-specific, the majority of these metals cause harm through similar processes, usually by substituting other elements in biochemical reactions or causing physiological alterations by binding to important enzymes

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The reaction mixture (1 ml) containing Lockes buffer (pH 7.4), 0.2 ml homogenate and 10 l of DCFH-DA (5 mM) was incubated for 45 min at room temperature and the fluorescence intensity of DCF was recorded by excitation at 485 nm and emission at 535 nm using a Synergy TM 2 Multi-function Microplate Reader (Bio-Tek Instruments Inc., Winooski, Vermont, USA)