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Description
The first obstacle encountered by circulating tumor cells is the blood vessel wall, especially endothelial cells
After 2 hours the media was removed and 100 l of DMSO was added to each well
The difference spectra were obtained by subtracting the dark spectrum from the illuminated spectrum

In the present study, the concentration of 200 pg/mL was assumed as a lower limit of the normal concentration of vitamin B 12 in blood serum
