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Pellets were thawed on ice and lysed in 2X lysis buffer (100 mM TrisHCl pH 7.5, 300 mM NaCl, 2 mM EDTA, 2% (v/v) IGEPAL CA-630, 1% sodium deoxycholate, 0.5 mM TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) with EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich) for 20 min on ice

Application: The treatment is applied in layers, allowing the active ingredients to penetrate deeply and target the areas of concern

On histopathology, 87 percent of the nevi were intradermal, 8 percent were compound nevi, and for 5 percent nevi the tissue was insufficient for diagnosis

Gas gangrene is an especially dangerous form because it spreads quickly throughout the body if left untreated, and can be fatal