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Aqueous humor samples from glaucoma patients Samples of human aqueous humor were obtained from glaucoma patients at the Department of Ophthalmology, Tohoku University Hospital

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After three washes in PBS, S-glutathionylated cysteine groups were reduced by incubation with 13.5 g/mL human Grx1 (Fitzgerald, 30R-1244), 35 g/mL GSSG reductase (Roche, 10105678001), 1 mM GSH (Sigma-Aldrich, G4251), 1 mM NADPH (Roche, 10107824001), 18 mol EDTA, and 137 mM TrisHCl, pH 8.0, for 20 min