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Then, membranes were incubated for 2 hours using primary antibodies for CYP 11A1, 21A2, and CYP 19 using the dilution of 1:1000 and then washed twice using Tween-TBS (0.2 ml Tween/ liter TBS) for 20 min, then with TBS for 15 min

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Forty-eight hours after transfection, 10 ml of supernatant was collected and filtered (0.45 m)

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