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72 h after transfection, cell supernatant was harvested and proteins were purified with Ni-NTA resin (Qiagen) followed by size-exclusion chromatography (SEC) on a Superdex 200 column (GE) in Phosphate-buffered saline (PBS, 135 mM NaCl, 2.5 mM KCl, 8.0 mM Na 2 HPO 4 , 30 mM KH 2 PO 4 , pH 7.2)
Compared to numerous studies conducted with synaptic NMDARs, few were done to extrasynaptic NMDARs and their function remains largely unknown until recently
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An HMGA2-IGF2BP2 Axis Regulates Myoblast Proliferation and Myogenesis
