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Cell suspensions were serially diluted and plated on TSA with 0.4% activated charcoal (Sigma) to neutralize residual antibiotics, allowing enumeration of surviving bacteria

Mouse Studies Rodent study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) at Baylor College of Medicine and adhered to the criteria outlined in the Guide of the Care and Use of Laboratory Animals

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Clinicians also consider side effects, including whether you tolerate daytime or nighttime dosing better, as well as your medications, health history, and other safety considerations