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Once activated, Ulk1 initiates autophagosome nucleation through the class III phosphatidylinositol 3-kinase (PtdIns3K) complex

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Bottom-up methods also include seed-mediated growth (growing larger nanostructures from smaller seeds), nanosphere lithography (using colloidal beads as masks), and chemical vapor deposition (CVD) for growing materials like graphene or nanowires