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HT1080 cells were cultured in Dulbeccos Modified Eagle Medium (DMEM) containing 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin solution at 37 C under a 5% CO 2 atmosphere

Current pharmaceutical design , 20 (7), 1126-35
Dzeranov A, Bondarenko L, Pankratov D, Prokofev M, Dzhardimalieva G, Jorobekova S, Tropskaya N, Telegina L, Kydralieva K (2023) Iron oxides nanoparticles as components of ferroptosis-inducing systems: screening of potential candidates

When benzodiazepines and opioids are combined, the potential for benzodiazepines to significantly worsen opioid-related respiratory depression exists