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Their findings revealed that GAG oligosaccharides with varying sulfation levels underwent ionization primarily as intact deprotonated singly charged ions, though doubly and even triply charged disaccharide ions could also be observed depending on sulfation degree, accompanied by minimal loss of sulfate groups

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The H-ESI source was operated in positive mode at spray voltage 3,500 or negative mode at spray voltage 2,500 with the following parameters: sheath gas 35 a.u., aux gas 7 a.u., sweep gas 0 a.u., ion transfer tube temperature 320 C, vaporizer temperature 275 C, mass range 70 to 1,000 m/z , full scan MS1 mass resolution of 120,000 FWHM, RF lens at 70% and standard AGC

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