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Bacteriostatic water containing 0.9% benzyl alcohol, lab-grade, sealed, and within its expiry date Sterile syringes , 3 mL or 5 mL, depending on your volume, with 21-gauge needles Sterile nasal spray bottle calibrated to deliver a fixed volume per actuation (typically 0.1 mL, confirm before starting) Alcohol prep pads , isopropyl at 70% concentration Nitrile gloves Clean, flat work surface that can be fully disinfected before use Critical: Confirm the actuation volume before you do any calculations

[DOI] [PubMed] [Google Scholar] 15.Campagnolo L, Costanza G, Francesconi A, et al

Khavinson and his collaborators have published the bulk of the existing research on it across roughly 25-30 years of work ranging from cell culture experiments through animal lifespan studies into human clinical observations

doi:10.1038/nchembio.1204